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Filtered Search Results
Thermo Scientific™ FastDigest NmuCI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest MbiI
Cut at CCGCTC(-3/-3)^ sites with Thermo Scientific™ FastDigest™ BsrBI (MbiI), which performs best using universal FastDigest Buffer (Isoschizomers: AccBSI).
Thermo Scientific™ BseGI (BtsCI) (10 U/μL)
Cut at GGATG(2/0)^ sites with BseGI (BtsCI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: BstF5I, BtsCI, FokI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | BseGI (BtsCI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Scarless Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | Yes |
| Optimal Reaction Temperature | 55°C |
| Concentration | 10 U/μL |
Thermo Scientific™ AdeI (DraIII) (10 U/μL)
Cut at CACNNN^GTG sites with AdeI (DraIII) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: DraIII).
Thermo Scientific™ HpyF10VI (MwoI) (10 U/μL)
The HpyF10VI (MwoI) restriction enzyme recognizes GCNNNNN^NNGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstMWI, MwoI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | HpyF10VI (MwoI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ XceI (NspI) (10 U/μL)
Cut at RCATG^Y sites with XceI (NspI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BstNSI, NspI).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ FastDigest SchI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ XmiI (AccI) (10 U/μL)
Cut at GT^MKAC sites with XmiI (AccI) restriction enzyme, which functions best at 37°C in B buffer (Isoschizomers: AccI, FblI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | XmiI (AccI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer B |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ SchI (MlyI) (10 U/μL)
Cut at GAGTC(5/5)^ sites with SchI (MlyI) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: MlyI, PleI, PpsI, SchI).
Thermo Scientific™ SfiI (10 U/μL)
Cut at GGCCNNNN^NGGCC sites with SfiI restriction enzyme, which performs best at 50°C in G buffer.
Thermo Scientific™ AlfI
Cut at ^(10/12)GCA(N)6TGC(12/10)^ sites with AlfI restriction enzyme, which performs best at 37°C in R (+SAM) buffer.
Thermo Scientific™ SatI (Fnu4HI) (10 U/μL)
The SatI (Fnu4HI) restriction enzyme recognizes GC^NGC sites and cuts best at 37°C in G buffer (Isoschizomers: Fnu4HI, Fsp4HI, ItaI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | SatI (Fnu4HI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer G |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest BfmI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ BseXI Buffer (10X)
Optimize digestions with BseXI restriction enzyme using this buffer, premixed with BSA for enhanced stability.
Thermo Scientific™ FastDigest Eco88I
Cut at C^YCGRG sites with Thermo Scientific™ FastDigest™ AvaI (Eco88I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.